All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer

Increased sensitivity of one-tube, quantitative RT-PCR.

With the introduction of real-time, fluorescence-based 5′ nuclease PCR (3,4) and instruments such as the ABI PRISM 7700 (TaqMan) sequence detector (Applied Biosystems, Foster City, CA, USA), quantitative RT-PCR is now a widely accepted method for measuring gene expression levels. Quantitative RTPCR is a sensitive technique and is particularly useful for the analysis of samples containing limi...

Quantitative tube model for semiflexible polymer solutions.

We develop an analytical and quantitative theory of the tube model concept for entangled networks of semiflexible polymers. The absolute value of the tube diameter L perpendicular is derived as a function of the polymers' persistence length lp and mesh size xi of the network. To leading order, we find L perpendicular = 0.31xi 6/5 lp (-1/5) , which is consistent with known asymptotic scaling law...

A CLOSED FORM SOLUTION FOR FREE VIBRATION ANALYSIS OF TUBE-IN-TUBE SYSTEMS IN TALL BUILDINGS

In this paper the dynamic response of tube-in-tube systems for tall building structures is investigated. Inner and outer tubes are modeled using equivalent continuous orthotropic membranes; in which, each tube is individually modeled by a cantilever box beam. By applying the compatibility conditions on deformation of the two tubes, the governing dynamic equations of the tube-in-tube structure a...

Stochastic tube MPC with state estimation

An output feedback Model Predictive Control (MPC) strategy for linear systems with additive stochastic disturbances and probabilistic constraints is proposed. Given the probability distributions of the disturbance input, the measurement noise and the initial state estimation error, the distributions of future realizations of the constrained variables are predicted using the dynamics of the plan...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)